Regulation of PfEMP1-VAR2CSA translation by a Plasmodium translation-enhancing factor

Research output: Contribution to journal › Journal article › Research › peer-review

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Regulation of PfEMP1-VAR2CSA translation by a Plasmodium translation-enhancing factor. / Chan, Sherwin; Frasch, Alejandra; Mandava, Chandra Sekhar; Ch'Ng, Jun Hong; Quintana, Maria Del Pilar; Vesterlund, Mattias; Ghorbal, Mehdi; Joannin, Nicolas; Franzén, Oscar; Lopez-Rubio, Jose Juan; Barbieri, Sonia; Lanzavecchia, Antonio; Sanyal, Suparna; Wahlgren, Mats.

In: Nature Microbiology, Vol. 2, 17068, 08.05.2017.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Chan, S, Frasch, A, Mandava, CS, Ch'Ng, JH, Quintana, MDP, Vesterlund, M, Ghorbal, M, Joannin, N, Franzén, O, Lopez-Rubio, JJ, Barbieri, S, Lanzavecchia, A, Sanyal, S & Wahlgren, M 2017, 'Regulation of PfEMP1-VAR2CSA translation by a Plasmodium translation-enhancing factor', Nature Microbiology, vol. 2, 17068. https://doi.org/10.1038/nmicrobiol.2017.68

APA

Chan, S., Frasch, A., Mandava, C. S., Ch'Ng, J. H., Quintana, M. D. P., Vesterlund, M., Ghorbal, M., Joannin, N., Franzén, O., Lopez-Rubio, J. J., Barbieri, S., Lanzavecchia, A., Sanyal, S., & Wahlgren, M. (2017). Regulation of PfEMP1-VAR2CSA translation by a Plasmodium translation-enhancing factor. Nature Microbiology, 2, [17068]. https://doi.org/10.1038/nmicrobiol.2017.68

Vancouver

Chan S, Frasch A, Mandava CS, Ch'Ng JH, Quintana MDP, Vesterlund M et al. Regulation of PfEMP1-VAR2CSA translation by a Plasmodium translation-enhancing factor. Nature Microbiology. 2017 May 8;2. 17068. https://doi.org/10.1038/nmicrobiol.2017.68

Author

Chan, Sherwin ; Frasch, Alejandra ; Mandava, Chandra Sekhar ; Ch'Ng, Jun Hong ; Quintana, Maria Del Pilar ; Vesterlund, Mattias ; Ghorbal, Mehdi ; Joannin, Nicolas ; Franzén, Oscar ; Lopez-Rubio, Jose Juan ; Barbieri, Sonia ; Lanzavecchia, Antonio ; Sanyal, Suparna ; Wahlgren, Mats. / Regulation of PfEMP1-VAR2CSA translation by a Plasmodium translation-enhancing factor. In: Nature Microbiology. 2017 ; Vol. 2.

Bibtex

@article{5985506d7c9743c1bdcaf82babb93cf7,
title = "Regulation of PfEMP1-VAR2CSA translation by a Plasmodium translation-enhancing factor",
abstract = "Pregnancy-associated malaria commonly involves the binding of Plasmodium falciparum-infected erythrocytes to placental chondroitin sulfate A (CSA) through the PfEMP1-VAR2CSA protein. VAR2CSA is translationally repressed by an upstream open reading frame. In this study, we report that the P. falciparum translation enhancing factor (PTEF) relieves upstream open reading frame repression and thereby facilitates VAR2CSA translation. VAR2CSA protein levels in var2csa-transcribing parasites are dependent on the expression level of PTEF, and the alleviation of upstream open reading frame repression requires the proteolytic processing of PTEF by PfCalpain. Cleavage generates a C-terminal domain that contains a sterile-alpha-motif-like domain. The C-terminal domain is permissive to cytoplasmic shuttling and interacts with ribosomes to facilitate translational derepression of the var2csa coding sequence. It also enhances translation in a heterologous translation system and thus represents the first non-canonical translation enhancing factor to be found in a protozoan. Our results implicate PTEF in regulating placental CSA binding of infected erythrocytes.",
author = "Sherwin Chan and Alejandra Frasch and Mandava, {Chandra Sekhar} and Ch'Ng, {Jun Hong} and Quintana, {Maria Del Pilar} and Mattias Vesterlund and Mehdi Ghorbal and Nicolas Joannin and Oscar Franz{\'e}n and Lopez-Rubio, {Jose Juan} and Sonia Barbieri and Antonio Lanzavecchia and Suparna Sanyal and Mats Wahlgren",
year = "2017",
month = may,
day = "8",
doi = "10.1038/nmicrobiol.2017.68",
language = "English",
volume = "2",
journal = "Nature Microbiology",
issn = "2058-5276",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Regulation of PfEMP1-VAR2CSA translation by a Plasmodium translation-enhancing factor

AU - Chan, Sherwin

AU - Frasch, Alejandra

AU - Mandava, Chandra Sekhar

AU - Ch'Ng, Jun Hong

AU - Quintana, Maria Del Pilar

AU - Vesterlund, Mattias

AU - Ghorbal, Mehdi

AU - Joannin, Nicolas

AU - Franzén, Oscar

AU - Lopez-Rubio, Jose Juan

AU - Barbieri, Sonia

AU - Lanzavecchia, Antonio

AU - Sanyal, Suparna

AU - Wahlgren, Mats

PY - 2017/5/8

Y1 - 2017/5/8

N2 - Pregnancy-associated malaria commonly involves the binding of Plasmodium falciparum-infected erythrocytes to placental chondroitin sulfate A (CSA) through the PfEMP1-VAR2CSA protein. VAR2CSA is translationally repressed by an upstream open reading frame. In this study, we report that the P. falciparum translation enhancing factor (PTEF) relieves upstream open reading frame repression and thereby facilitates VAR2CSA translation. VAR2CSA protein levels in var2csa-transcribing parasites are dependent on the expression level of PTEF, and the alleviation of upstream open reading frame repression requires the proteolytic processing of PTEF by PfCalpain. Cleavage generates a C-terminal domain that contains a sterile-alpha-motif-like domain. The C-terminal domain is permissive to cytoplasmic shuttling and interacts with ribosomes to facilitate translational derepression of the var2csa coding sequence. It also enhances translation in a heterologous translation system and thus represents the first non-canonical translation enhancing factor to be found in a protozoan. Our results implicate PTEF in regulating placental CSA binding of infected erythrocytes.

AB - Pregnancy-associated malaria commonly involves the binding of Plasmodium falciparum-infected erythrocytes to placental chondroitin sulfate A (CSA) through the PfEMP1-VAR2CSA protein. VAR2CSA is translationally repressed by an upstream open reading frame. In this study, we report that the P. falciparum translation enhancing factor (PTEF) relieves upstream open reading frame repression and thereby facilitates VAR2CSA translation. VAR2CSA protein levels in var2csa-transcribing parasites are dependent on the expression level of PTEF, and the alleviation of upstream open reading frame repression requires the proteolytic processing of PTEF by PfCalpain. Cleavage generates a C-terminal domain that contains a sterile-alpha-motif-like domain. The C-terminal domain is permissive to cytoplasmic shuttling and interacts with ribosomes to facilitate translational derepression of the var2csa coding sequence. It also enhances translation in a heterologous translation system and thus represents the first non-canonical translation enhancing factor to be found in a protozoan. Our results implicate PTEF in regulating placental CSA binding of infected erythrocytes.

U2 - 10.1038/nmicrobiol.2017.68

DO - 10.1038/nmicrobiol.2017.68

M3 - Journal article

C2 - 28481333

AN - SCOPUS:85019133411

VL - 2

JO - Nature Microbiology

JF - Nature Microbiology

SN - 2058-5276

M1 - 17068

ER -

ID: 197729158