Molecular surveillance of the antifolate-resistant mutation I164L in imported African isolates of Plasmodium falciparum in Europe: sentinel data from TropNetEurop
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Molecular surveillance of the antifolate-resistant mutation I164L in imported African isolates of Plasmodium falciparum in Europe: sentinel data from TropNetEurop. / Wichmann, Ole; Jelinek, Tomas; Peyerl-Hoffmann, Gabriele; Mühlberger, Nikolai; Grobusch, Martin P; Gascon, Joaquim; Matteelli, Alberto; Hatz, Christoph; Laferl, Hermann; Schulze, Marco; Burchard, Gerd; da Cunha, Saraiva; Beran, Jiøi; McWhinney, Paul; Kollaritsch, Herwig; Kern, Peter; Cuadros, Juan; Alifrangis, Michael; Gjørup, Ida; European Network on Surveillance of Imported Infectious Diseases (TropNetEurop).
In: Malaria Journal, Vol. 2, 2003, p. 17.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Molecular surveillance of the antifolate-resistant mutation I164L in imported African isolates of Plasmodium falciparum in Europe: sentinel data from TropNetEurop
AU - Wichmann, Ole
AU - Jelinek, Tomas
AU - Peyerl-Hoffmann, Gabriele
AU - Mühlberger, Nikolai
AU - Grobusch, Martin P
AU - Gascon, Joaquim
AU - Matteelli, Alberto
AU - Hatz, Christoph
AU - Laferl, Hermann
AU - Schulze, Marco
AU - Burchard, Gerd
AU - da Cunha, Saraiva
AU - Beran, Jiøi
AU - McWhinney, Paul
AU - Kollaritsch, Herwig
AU - Kern, Peter
AU - Cuadros, Juan
AU - Alifrangis, Michael
AU - Gjørup, Ida
AU - European Network on Surveillance of Imported Infectious Diseases (TropNetEurop)
N1 - Keywords: Amino Acid Substitution; Animals; Drug Resistance, Multiple; Folic Acid Antagonists; Humans; Isoleucine; Leucine; Malaria, Falciparum; Plasmodium falciparum; Population Surveillance
PY - 2003
Y1 - 2003
N2 - BACKGROUND: Malaria parasites that carry the DHFR-mutation I164L are not only highly resistant to sulfadoxine-pyrimethamine but also to the new antimalarial drug chlorproguanil-dapsone. The spread of this mutation in Africa would result in a public health disaster since there is a lack of effective alternatives that are both affordable and safe. Up to now, this mutation has only been described in Asian and Latin-American countries. The objective of this study was to assess the prevalence of this mutation in African isolates of Plasmodium falciparum that have been imported into Europe through travellers. METHODS: TropNetEurop is a network for the surveillance of travel-associated diseases and seems to cover approximately 12% of all malaria cases imported into Europe. Within this network we screened 277 imported African isolates of P. falciparum with the help of PCR- and enzyme-digestion-methods for the antifolate-resistant mutation I164L. RESULTS: The I164L mutation was not detected in any of the isolates tested. DISCUSSION: Continuous molecular surveillance of mutations in P. falciparum, as it is practised within TropNetEurop, is an essential tool for the understanding and early detection of the spread of antimalarial drug resistance in Africa.
AB - BACKGROUND: Malaria parasites that carry the DHFR-mutation I164L are not only highly resistant to sulfadoxine-pyrimethamine but also to the new antimalarial drug chlorproguanil-dapsone. The spread of this mutation in Africa would result in a public health disaster since there is a lack of effective alternatives that are both affordable and safe. Up to now, this mutation has only been described in Asian and Latin-American countries. The objective of this study was to assess the prevalence of this mutation in African isolates of Plasmodium falciparum that have been imported into Europe through travellers. METHODS: TropNetEurop is a network for the surveillance of travel-associated diseases and seems to cover approximately 12% of all malaria cases imported into Europe. Within this network we screened 277 imported African isolates of P. falciparum with the help of PCR- and enzyme-digestion-methods for the antifolate-resistant mutation I164L. RESULTS: The I164L mutation was not detected in any of the isolates tested. DISCUSSION: Continuous molecular surveillance of mutations in P. falciparum, as it is practised within TropNetEurop, is an essential tool for the understanding and early detection of the spread of antimalarial drug resistance in Africa.
U2 - 10.1186/1475-2875-2-17
DO - 10.1186/1475-2875-2-17
M3 - Journal article
C2 - 12869209
VL - 2
SP - 17
JO - Malaria Journal
JF - Malaria Journal
SN - 1475-2875
ER -
ID: 8377953