Th1-like human T-cell clones recognizing Leishmania gp63 inhibit Leishmania major in human macrophages

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Th1-like human T-cell clones recognizing Leishmania gp63 inhibit Leishmania major in human macrophages. / Kemp, M; Hey, A S; Bendtzen, K; Kharazmi, A; Theander, T G.

In: Scandinavian Journal of Immunology, Vol. 40, No. 6, 1994, p. 629-35.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kemp, M, Hey, AS, Bendtzen, K, Kharazmi, A & Theander, TG 1994, 'Th1-like human T-cell clones recognizing Leishmania gp63 inhibit Leishmania major in human macrophages', Scandinavian Journal of Immunology, vol. 40, no. 6, pp. 629-35.

APA

Kemp, M., Hey, A. S., Bendtzen, K., Kharazmi, A., & Theander, T. G. (1994). Th1-like human T-cell clones recognizing Leishmania gp63 inhibit Leishmania major in human macrophages. Scandinavian Journal of Immunology, 40(6), 629-35.

Vancouver

Kemp M, Hey AS, Bendtzen K, Kharazmi A, Theander TG. Th1-like human T-cell clones recognizing Leishmania gp63 inhibit Leishmania major in human macrophages. Scandinavian Journal of Immunology. 1994;40(6):629-35.

Author

Kemp, M ; Hey, A S ; Bendtzen, K ; Kharazmi, A ; Theander, T G. / Th1-like human T-cell clones recognizing Leishmania gp63 inhibit Leishmania major in human macrophages. In: Scandinavian Journal of Immunology. 1994 ; Vol. 40, No. 6. pp. 629-35.

Bibtex

@article{4ff76170a0da11dd86a6000ea68e967b,
title = "Th1-like human T-cell clones recognizing Leishmania gp63 inhibit Leishmania major in human macrophages",
abstract = "The major surface protease of Leishmania major, gp63, has been suggested as a vaccine candidate for cutaneous leishmaniasis. In this study gp63 was purified from L. major promastigotes. A panel of human T-cell clones recognizing this protein were generated from individuals who had previously had self-healing cutaneous leishmaniasis. The T-cell clones expressed CD4, and the alpha chain of the T-cell antigen receptor. GP63 reactive T-cell clones activated by antigen or by immobilized anti-CD3 antibody released relative large amounts of interferon-gamma and no or little interleukin-4, thereby resembling Th1 cells. Autologous mononuclear cells and Epstein-Barr virus-transformed B cell lines were equally efficient in presenting the antigen to the T cells. The gp63 reactive T cells induced resistance to infection in cultured human macrophages by L. major. The data confirm that human CD4+ T cells recognizing gp63 can take part in the host defence against L. major infections.",
author = "M Kemp and Hey, {A S} and K Bendtzen and A Kharazmi and Theander, {T G}",
note = "Keywords: Adult; Animals; Antigens, Protozoan; Clone Cells; Flow Cytometry; Humans; Interferon Type II; Interleukin-4; Leishmania major; Macrophages; Metalloendopeptidases; Th1 Cells",
year = "1994",
language = "English",
volume = "40",
pages = "629--35",
journal = "Scandinavian Journal of Immunology, Supplement",
issn = "0301-6323",
publisher = "Wiley-Blackwell",
number = "6",

}

RIS

TY - JOUR

T1 - Th1-like human T-cell clones recognizing Leishmania gp63 inhibit Leishmania major in human macrophages

AU - Kemp, M

AU - Hey, A S

AU - Bendtzen, K

AU - Kharazmi, A

AU - Theander, T G

N1 - Keywords: Adult; Animals; Antigens, Protozoan; Clone Cells; Flow Cytometry; Humans; Interferon Type II; Interleukin-4; Leishmania major; Macrophages; Metalloendopeptidases; Th1 Cells

PY - 1994

Y1 - 1994

N2 - The major surface protease of Leishmania major, gp63, has been suggested as a vaccine candidate for cutaneous leishmaniasis. In this study gp63 was purified from L. major promastigotes. A panel of human T-cell clones recognizing this protein were generated from individuals who had previously had self-healing cutaneous leishmaniasis. The T-cell clones expressed CD4, and the alpha chain of the T-cell antigen receptor. GP63 reactive T-cell clones activated by antigen or by immobilized anti-CD3 antibody released relative large amounts of interferon-gamma and no or little interleukin-4, thereby resembling Th1 cells. Autologous mononuclear cells and Epstein-Barr virus-transformed B cell lines were equally efficient in presenting the antigen to the T cells. The gp63 reactive T cells induced resistance to infection in cultured human macrophages by L. major. The data confirm that human CD4+ T cells recognizing gp63 can take part in the host defence against L. major infections.

AB - The major surface protease of Leishmania major, gp63, has been suggested as a vaccine candidate for cutaneous leishmaniasis. In this study gp63 was purified from L. major promastigotes. A panel of human T-cell clones recognizing this protein were generated from individuals who had previously had self-healing cutaneous leishmaniasis. The T-cell clones expressed CD4, and the alpha chain of the T-cell antigen receptor. GP63 reactive T-cell clones activated by antigen or by immobilized anti-CD3 antibody released relative large amounts of interferon-gamma and no or little interleukin-4, thereby resembling Th1 cells. Autologous mononuclear cells and Epstein-Barr virus-transformed B cell lines were equally efficient in presenting the antigen to the T cells. The gp63 reactive T cells induced resistance to infection in cultured human macrophages by L. major. The data confirm that human CD4+ T cells recognizing gp63 can take part in the host defence against L. major infections.

M3 - Journal article

C2 - 7997852

VL - 40

SP - 629

EP - 635

JO - Scandinavian Journal of Immunology, Supplement

JF - Scandinavian Journal of Immunology, Supplement

SN - 0301-6323

IS - 6

ER -

ID: 6766552