Soluble Plasmodium falciparum antigens contain carbohydrate moieties important for immune reactivity
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Soluble Plasmodium falciparum antigens contain carbohydrate moieties important for immune reactivity. / Jakobsen, P H; Theander, T G; Jensen, J B; Mølbak, K; Jepsen, S.
In: Journal of Clinical Microbiology, Vol. 25, No. 11, 1987, p. 2075-9.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Soluble Plasmodium falciparum antigens contain carbohydrate moieties important for immune reactivity
AU - Jakobsen, P H
AU - Theander, T G
AU - Jensen, J B
AU - Mølbak, K
AU - Jepsen, S
N1 - Keywords: Animals; Antigens, Protozoan; Carbohydrates; Humans; Immunoassay; Immunoelectrophoresis, Two-Dimensional; Leukocytes, Mononuclear; Malaria; Plasmodium falciparum; alpha-Galactosidase
PY - 1987
Y1 - 1987
N2 - The importance of carbohydrate moieties for the antigenicity of purified soluble Plasmodium falciparum antigens from the asexual blood stage was tested. Digestion of the soluble antigens with alpha-D-galactosidase clearly affected the ability of the antigen to react with malaria-immune sera from different geographical origins in crossed immunoelectrophoresis and immunoblotting. Antigens of 220, 180, 80, and 74 kilodaltons were affected by the enzyme treatment. Furthermore, the enzyme digestion reduced the ability of the purified soluble antigen to stimulate lymphocytes from malaria-immune donors. The results might have important implications for the strategy of developing a malaria vaccine.
AB - The importance of carbohydrate moieties for the antigenicity of purified soluble Plasmodium falciparum antigens from the asexual blood stage was tested. Digestion of the soluble antigens with alpha-D-galactosidase clearly affected the ability of the antigen to react with malaria-immune sera from different geographical origins in crossed immunoelectrophoresis and immunoblotting. Antigens of 220, 180, 80, and 74 kilodaltons were affected by the enzyme treatment. Furthermore, the enzyme digestion reduced the ability of the purified soluble antigen to stimulate lymphocytes from malaria-immune donors. The results might have important implications for the strategy of developing a malaria vaccine.
M3 - Journal article
C2 - 2826533
VL - 25
SP - 2075
EP - 2079
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 11
ER -
ID: 6767067