Real-time and label free determination of ligand binding-kinetics to primary cancer tissue specimens; a novel tool for the assessment of biomarker targeting

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Real-time and label free determination of ligand binding-kinetics to primary cancer tissue specimens; a novel tool for the assessment of biomarker targeting. / Clausen, Thomas Mandel; Ayres Pereira, Marina; Oo, Htoo Zarni; Resende, Mafalda; Gustavson, Tobias; Mao, Yang; Sugiura, Nobuo; Liew, Janet; Fazli, Ladan; Theander, Thor G; Daugaard, Mads; Salanti, Ali.

In: Sensing and Bio-Sensing Research, Vol. 9, 07.2016, p. 23-30.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Clausen, TM, Ayres Pereira, M, Oo, HZ, Resende, M, Gustavson, T, Mao, Y, Sugiura, N, Liew, J, Fazli, L, Theander, TG, Daugaard, M & Salanti, A 2016, 'Real-time and label free determination of ligand binding-kinetics to primary cancer tissue specimens; a novel tool for the assessment of biomarker targeting', Sensing and Bio-Sensing Research, vol. 9, pp. 23-30. https://doi.org/10.1016/j.sbsr.2016.05.003

APA

Clausen, T. M., Ayres Pereira, M., Oo, H. Z., Resende, M., Gustavson, T., Mao, Y., Sugiura, N., Liew, J., Fazli, L., Theander, T. G., Daugaard, M., & Salanti, A. (2016). Real-time and label free determination of ligand binding-kinetics to primary cancer tissue specimens; a novel tool for the assessment of biomarker targeting. Sensing and Bio-Sensing Research, 9, 23-30. https://doi.org/10.1016/j.sbsr.2016.05.003

Vancouver

Clausen TM, Ayres Pereira M, Oo HZ, Resende M, Gustavson T, Mao Y et al. Real-time and label free determination of ligand binding-kinetics to primary cancer tissue specimens; a novel tool for the assessment of biomarker targeting. Sensing and Bio-Sensing Research. 2016 Jul;9:23-30. https://doi.org/10.1016/j.sbsr.2016.05.003

Author

Clausen, Thomas Mandel ; Ayres Pereira, Marina ; Oo, Htoo Zarni ; Resende, Mafalda ; Gustavson, Tobias ; Mao, Yang ; Sugiura, Nobuo ; Liew, Janet ; Fazli, Ladan ; Theander, Thor G ; Daugaard, Mads ; Salanti, Ali. / Real-time and label free determination of ligand binding-kinetics to primary cancer tissue specimens; a novel tool for the assessment of biomarker targeting. In: Sensing and Bio-Sensing Research. 2016 ; Vol. 9. pp. 23-30.

Bibtex

@article{be290d2bb40b401e98b88be207921d72,
title = "Real-time and label free determination of ligand binding-kinetics to primary cancer tissue specimens; a novel tool for the assessment of biomarker targeting",
abstract = "In clinical oncology, diagnosis and evaluation of optimal treatment strategies are mostly based on histopathological examination combined with immunohistochemical (IHC) expression analysis of cancer-associated antigens in formalin fixed paraffin-embedded (FFPE) tissue biopsies. However, informative IHC analysis depends on both the specificity and affinity of the binding reagent, which are inherently difficult to quantify in situ. Here we describe a label-free method that allows for the direct and real-time assessment of molecular binding kinetics in situ on FFPE tissue specimens using quartz crystal microbalance (QCM) enabled biosensor technology. We analysed the interaction between the rVAR2 protein and its placental-like chondroitin sulfate (pl-CS) receptor in primary human placenta tissue and in breast and prostate tumour specimens in situ. rVAR2 interacted with FFPE human placenta and cancer tissue with an affinity in the nanomolar range, and showed no detectable interaction with pl-CS negative normal tissue. We further validated the method by including analysis with the androgen receptor N-20 antibody (anti-AR). As the KD value produced by this method is independent of the number of epitopes available, this readout offers a quantitative and unbiased readout for in situ binding-avidity and amount of binding epitopes. In summary, this method adds a new and important dimension to classical IHC-based molecular pathology by adding information about the binding characteristics in biologically relevant conditions. This can potentially be used to select optimal biologics for diagnostic and for therapeutic applications as well as guide the development of novel high affinity binding drugs.",
author = "Clausen, {Thomas Mandel} and {Ayres Pereira}, Marina and Oo, {Htoo Zarni} and Mafalda Resende and Tobias Gustavson and Yang Mao and Nobuo Sugiura and Janet Liew and Ladan Fazli and Theander, {Thor G} and Mads Daugaard and Ali Salanti",
year = "2016",
month = jul,
doi = "10.1016/j.sbsr.2016.05.003",
language = "English",
volume = "9",
pages = "23--30",
journal = "Sensing and Bio-Sensing Research",
issn = "2214-1804",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Real-time and label free determination of ligand binding-kinetics to primary cancer tissue specimens; a novel tool for the assessment of biomarker targeting

AU - Clausen, Thomas Mandel

AU - Ayres Pereira, Marina

AU - Oo, Htoo Zarni

AU - Resende, Mafalda

AU - Gustavson, Tobias

AU - Mao, Yang

AU - Sugiura, Nobuo

AU - Liew, Janet

AU - Fazli, Ladan

AU - Theander, Thor G

AU - Daugaard, Mads

AU - Salanti, Ali

PY - 2016/7

Y1 - 2016/7

N2 - In clinical oncology, diagnosis and evaluation of optimal treatment strategies are mostly based on histopathological examination combined with immunohistochemical (IHC) expression analysis of cancer-associated antigens in formalin fixed paraffin-embedded (FFPE) tissue biopsies. However, informative IHC analysis depends on both the specificity and affinity of the binding reagent, which are inherently difficult to quantify in situ. Here we describe a label-free method that allows for the direct and real-time assessment of molecular binding kinetics in situ on FFPE tissue specimens using quartz crystal microbalance (QCM) enabled biosensor technology. We analysed the interaction between the rVAR2 protein and its placental-like chondroitin sulfate (pl-CS) receptor in primary human placenta tissue and in breast and prostate tumour specimens in situ. rVAR2 interacted with FFPE human placenta and cancer tissue with an affinity in the nanomolar range, and showed no detectable interaction with pl-CS negative normal tissue. We further validated the method by including analysis with the androgen receptor N-20 antibody (anti-AR). As the KD value produced by this method is independent of the number of epitopes available, this readout offers a quantitative and unbiased readout for in situ binding-avidity and amount of binding epitopes. In summary, this method adds a new and important dimension to classical IHC-based molecular pathology by adding information about the binding characteristics in biologically relevant conditions. This can potentially be used to select optimal biologics for diagnostic and for therapeutic applications as well as guide the development of novel high affinity binding drugs.

AB - In clinical oncology, diagnosis and evaluation of optimal treatment strategies are mostly based on histopathological examination combined with immunohistochemical (IHC) expression analysis of cancer-associated antigens in formalin fixed paraffin-embedded (FFPE) tissue biopsies. However, informative IHC analysis depends on both the specificity and affinity of the binding reagent, which are inherently difficult to quantify in situ. Here we describe a label-free method that allows for the direct and real-time assessment of molecular binding kinetics in situ on FFPE tissue specimens using quartz crystal microbalance (QCM) enabled biosensor technology. We analysed the interaction between the rVAR2 protein and its placental-like chondroitin sulfate (pl-CS) receptor in primary human placenta tissue and in breast and prostate tumour specimens in situ. rVAR2 interacted with FFPE human placenta and cancer tissue with an affinity in the nanomolar range, and showed no detectable interaction with pl-CS negative normal tissue. We further validated the method by including analysis with the androgen receptor N-20 antibody (anti-AR). As the KD value produced by this method is independent of the number of epitopes available, this readout offers a quantitative and unbiased readout for in situ binding-avidity and amount of binding epitopes. In summary, this method adds a new and important dimension to classical IHC-based molecular pathology by adding information about the binding characteristics in biologically relevant conditions. This can potentially be used to select optimal biologics for diagnostic and for therapeutic applications as well as guide the development of novel high affinity binding drugs.

U2 - 10.1016/j.sbsr.2016.05.003

DO - 10.1016/j.sbsr.2016.05.003

M3 - Journal article

C2 - 27441183

VL - 9

SP - 23

EP - 30

JO - Sensing and Bio-Sensing Research

JF - Sensing and Bio-Sensing Research

SN - 2214-1804

ER -

ID: 164856397