Immunomagnetic Selection of Plasmodium falciparum–Infected Erythrocytes Expressing Particular PfEMP1 Variants

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Immunomagnetic Selection of Plasmodium falciparum–Infected Erythrocytes Expressing Particular PfEMP1 Variants. / Lopez-Perez, Mary; Olsen, Rebecca Wendelboe.

Methods in Molecular Biology: Targeting the Surface of Infected Erythrocytes. Humana Press, 2022. p. 69-78 (Methods in Molecular Biology, Vol. 2470).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Lopez-Perez, M & Olsen, RW 2022, Immunomagnetic Selection of Plasmodium falciparum–Infected Erythrocytes Expressing Particular PfEMP1 Variants. in Methods in Molecular Biology: Targeting the Surface of Infected Erythrocytes. Humana Press, Methods in Molecular Biology, vol. 2470, pp. 69-78. https://doi.org/10.1007/978-1-0716-2189-9_7

APA

Lopez-Perez, M., & Olsen, R. W. (2022). Immunomagnetic Selection of Plasmodium falciparum–Infected Erythrocytes Expressing Particular PfEMP1 Variants. In Methods in Molecular Biology: Targeting the Surface of Infected Erythrocytes (pp. 69-78). Humana Press. Methods in Molecular Biology Vol. 2470 https://doi.org/10.1007/978-1-0716-2189-9_7

Vancouver

Lopez-Perez M, Olsen RW. Immunomagnetic Selection of Plasmodium falciparum–Infected Erythrocytes Expressing Particular PfEMP1 Variants. In Methods in Molecular Biology: Targeting the Surface of Infected Erythrocytes. Humana Press. 2022. p. 69-78. (Methods in Molecular Biology, Vol. 2470). https://doi.org/10.1007/978-1-0716-2189-9_7

Author

Lopez-Perez, Mary ; Olsen, Rebecca Wendelboe. / Immunomagnetic Selection of Plasmodium falciparum–Infected Erythrocytes Expressing Particular PfEMP1 Variants. Methods in Molecular Biology: Targeting the Surface of Infected Erythrocytes. Humana Press, 2022. pp. 69-78 (Methods in Molecular Biology, Vol. 2470).

Bibtex

@inbook{771ad9a3c72f400c928b45682a2fe469,
title = "Immunomagnetic Selection of Plasmodium falciparum–Infected Erythrocytes Expressing Particular PfEMP1 Variants",
abstract = "Plasmodium falciparum expresses a broad range of proteins on the surface of infected erythrocytes (IEs), including members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. This protocol describes an immunomagnetic selection method using PfEMP1-specific antibodies to obtain a parasite clone homogenously expressing a particular PfEMP1 protein. The expression of the corresponding PfEMP1 is later tested by flow cytometry, and the selected parasites can be used for further analysis.",
keywords = "Antibodies, Antiserum, Flow cytometry, Immunomagnetic selection, Magnetic beads, PfEMP1",
author = "Mary Lopez-Perez and Olsen, {Rebecca Wendelboe}",
note = "Funding Information: The authors{\textquoteright} research work is supported by the Danish International Development Agency—Danida (17-02-KU). Maiken Visti and Mette Madsen are thanked for their technical assistance. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_7",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "69--78",
booktitle = "Methods in Molecular Biology",
address = "United States",

}

RIS

TY - CHAP

T1 - Immunomagnetic Selection of Plasmodium falciparum–Infected Erythrocytes Expressing Particular PfEMP1 Variants

AU - Lopez-Perez, Mary

AU - Olsen, Rebecca Wendelboe

N1 - Funding Information: The authors’ research work is supported by the Danish International Development Agency—Danida (17-02-KU). Maiken Visti and Mette Madsen are thanked for their technical assistance. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - Plasmodium falciparum expresses a broad range of proteins on the surface of infected erythrocytes (IEs), including members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. This protocol describes an immunomagnetic selection method using PfEMP1-specific antibodies to obtain a parasite clone homogenously expressing a particular PfEMP1 protein. The expression of the corresponding PfEMP1 is later tested by flow cytometry, and the selected parasites can be used for further analysis.

AB - Plasmodium falciparum expresses a broad range of proteins on the surface of infected erythrocytes (IEs), including members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. This protocol describes an immunomagnetic selection method using PfEMP1-specific antibodies to obtain a parasite clone homogenously expressing a particular PfEMP1 protein. The expression of the corresponding PfEMP1 is later tested by flow cytometry, and the selected parasites can be used for further analysis.

KW - Antibodies

KW - Antiserum

KW - Flow cytometry

KW - Immunomagnetic selection

KW - Magnetic beads

KW - PfEMP1

U2 - 10.1007/978-1-0716-2189-9_7

DO - 10.1007/978-1-0716-2189-9_7

M3 - Book chapter

C2 - 35881339

AN - SCOPUS:85135381612

T3 - Methods in Molecular Biology

SP - 69

EP - 78

BT - Methods in Molecular Biology

PB - Humana Press

ER -

ID: 320006441