Electrical wiring of live, metabolically enhanced Bacillus subtilis cells with flexible osmium-redox polymers

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Electrical wiring of live, metabolically enhanced Bacillus subtilis cells with flexible osmium-redox polymers. / Coman, Vasile; Gustavsson, Tobias; Finkelsteinas, Arnonas; von Wachenfeldt, Claes; Hägerhäll, Cecilia; Gorton, Lo.

In: Journal of the American Chemical Society, Vol. 131, No. 44, 11.11.2009, p. 16171-6.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Coman, V, Gustavsson, T, Finkelsteinas, A, von Wachenfeldt, C, Hägerhäll, C & Gorton, L 2009, 'Electrical wiring of live, metabolically enhanced Bacillus subtilis cells with flexible osmium-redox polymers', Journal of the American Chemical Society, vol. 131, no. 44, pp. 16171-6. https://doi.org/10.1021/ja905442a

APA

Coman, V., Gustavsson, T., Finkelsteinas, A., von Wachenfeldt, C., Hägerhäll, C., & Gorton, L. (2009). Electrical wiring of live, metabolically enhanced Bacillus subtilis cells with flexible osmium-redox polymers. Journal of the American Chemical Society, 131(44), 16171-6. https://doi.org/10.1021/ja905442a

Vancouver

Coman V, Gustavsson T, Finkelsteinas A, von Wachenfeldt C, Hägerhäll C, Gorton L. Electrical wiring of live, metabolically enhanced Bacillus subtilis cells with flexible osmium-redox polymers. Journal of the American Chemical Society. 2009 Nov 11;131(44):16171-6. https://doi.org/10.1021/ja905442a

Author

Coman, Vasile ; Gustavsson, Tobias ; Finkelsteinas, Arnonas ; von Wachenfeldt, Claes ; Hägerhäll, Cecilia ; Gorton, Lo. / Electrical wiring of live, metabolically enhanced Bacillus subtilis cells with flexible osmium-redox polymers. In: Journal of the American Chemical Society. 2009 ; Vol. 131, No. 44. pp. 16171-6.

Bibtex

@article{0ca5c8e0b06946008a0228262a182026,
title = "Electrical wiring of live, metabolically enhanced Bacillus subtilis cells with flexible osmium-redox polymers",
abstract = "The present study explores genetic engineering of the respiratory chain and the application of two different flexible osmium redox polymers to achieve efficient electric communication between the gram-positive organism Bacillus subtilis and an electrode. Poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-bipyridyl)(2)Cl(2)](+/2+) (osmium redox polymer I) and poly(vinylpyridine)-[Os-(N,N'-methylated-2,2'-biimidazole)(3)](2+/3+) (osmium redox polymer II) were investigated for efficient electrical {"}wiring{"} of viable gram-positive bacterial cells to electrodes. Using a B. subtilis strain that overproduces succinate/quinone oxidoreductase (respiratory complex II), we were able to improve the current response several fold using succinate as substrate, in both batch and flow analysis modes, and using gold and graphite electrodes. The efficiency of the osmium redox polymer, working as electron transfer mediator between the cells and the electrode, was compared with that of a soluble mediator (hexacyanoferrate). The results demonstrated that mediators did not have to pass the cytosolic membrane to bring about an efficient electronic communication between bacterial cells with a thick cell wall and electrodes.",
keywords = "Bacillus subtilis/cytology, Electrochemical Techniques, Electrodes, Electron Transport Complex II/genetics, Genetic Engineering, Osmium, Polymers",
author = "Vasile Coman and Tobias Gustavsson and Arnonas Finkelsteinas and {von Wachenfeldt}, Claes and Cecilia H{\"a}gerh{\"a}ll and Lo Gorton",
year = "2009",
month = nov,
day = "11",
doi = "10.1021/ja905442a",
language = "English",
volume = "131",
pages = "16171--6",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "ACS Publications",
number = "44",

}

RIS

TY - JOUR

T1 - Electrical wiring of live, metabolically enhanced Bacillus subtilis cells with flexible osmium-redox polymers

AU - Coman, Vasile

AU - Gustavsson, Tobias

AU - Finkelsteinas, Arnonas

AU - von Wachenfeldt, Claes

AU - Hägerhäll, Cecilia

AU - Gorton, Lo

PY - 2009/11/11

Y1 - 2009/11/11

N2 - The present study explores genetic engineering of the respiratory chain and the application of two different flexible osmium redox polymers to achieve efficient electric communication between the gram-positive organism Bacillus subtilis and an electrode. Poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-bipyridyl)(2)Cl(2)](+/2+) (osmium redox polymer I) and poly(vinylpyridine)-[Os-(N,N'-methylated-2,2'-biimidazole)(3)](2+/3+) (osmium redox polymer II) were investigated for efficient electrical "wiring" of viable gram-positive bacterial cells to electrodes. Using a B. subtilis strain that overproduces succinate/quinone oxidoreductase (respiratory complex II), we were able to improve the current response several fold using succinate as substrate, in both batch and flow analysis modes, and using gold and graphite electrodes. The efficiency of the osmium redox polymer, working as electron transfer mediator between the cells and the electrode, was compared with that of a soluble mediator (hexacyanoferrate). The results demonstrated that mediators did not have to pass the cytosolic membrane to bring about an efficient electronic communication between bacterial cells with a thick cell wall and electrodes.

AB - The present study explores genetic engineering of the respiratory chain and the application of two different flexible osmium redox polymers to achieve efficient electric communication between the gram-positive organism Bacillus subtilis and an electrode. Poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-bipyridyl)(2)Cl(2)](+/2+) (osmium redox polymer I) and poly(vinylpyridine)-[Os-(N,N'-methylated-2,2'-biimidazole)(3)](2+/3+) (osmium redox polymer II) were investigated for efficient electrical "wiring" of viable gram-positive bacterial cells to electrodes. Using a B. subtilis strain that overproduces succinate/quinone oxidoreductase (respiratory complex II), we were able to improve the current response several fold using succinate as substrate, in both batch and flow analysis modes, and using gold and graphite electrodes. The efficiency of the osmium redox polymer, working as electron transfer mediator between the cells and the electrode, was compared with that of a soluble mediator (hexacyanoferrate). The results demonstrated that mediators did not have to pass the cytosolic membrane to bring about an efficient electronic communication between bacterial cells with a thick cell wall and electrodes.

KW - Bacillus subtilis/cytology

KW - Electrochemical Techniques

KW - Electrodes

KW - Electron Transport Complex II/genetics

KW - Genetic Engineering

KW - Osmium

KW - Polymers

U2 - 10.1021/ja905442a

DO - 10.1021/ja905442a

M3 - Journal article

C2 - 19886699

VL - 131

SP - 16171

EP - 16176

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 44

ER -

ID: 201046549