Cytokine production and apoptosis among T cells from patients under treatment for Plasmodium falciparum malaria
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Cytokine production and apoptosis among T cells from patients under treatment for Plasmodium falciparum malaria. / Kemp, K; Akanmori, B D; Adabayeri, V; Goka, B Q; Kurtzhals, J A L; Behr, C; Hviid, L.
In: Clinical and Experimental Immunology, Vol. 127, No. 1, 2002, p. 151-7.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cytokine production and apoptosis among T cells from patients under treatment for Plasmodium falciparum malaria
AU - Kemp, K
AU - Akanmori, B D
AU - Adabayeri, V
AU - Goka, B Q
AU - Kurtzhals, J A L
AU - Behr, C
AU - Hviid, L
N1 - Keywords: Animals; Antimalarials; Apoptosis; Child; Child, Preschool; Cytokines; Humans; Lymphocyte Activation; Malaria, Falciparum; Plasmodium falciparum; T-Lymphocyte Subsets
PY - 2002
Y1 - 2002
N2 - Available evidence suggests that Plasmodium falciparum malaria causes activation and reallocation of T cells, and that these in vivo primed cells re-emerge into the periphery following drug therapy. Here we have examined the cytokine production capacity and susceptibility to programmed cell death of peripheral T cells during and after the period of antimalarial treatment. A high proportion of peripheral CD3+ cells had an activated phenotype at and shortly after time of admission (day 0) and initiation of therapy. This activation peaked around day 2, and at this time-point peripheral T cells from the patients could be induced to produce cytokines at conditions of limited cytokine response in cells from healthy control donors. Activated CD8hi and TCR-gammadelta+ cells were the primary IFN-gamma producers, whereas CD4+ cells constituted an important source of TNF-alpha. The proportion of apoptotic T cells was elevated at admission and peaked 2 days later, while susceptibility to activation-induced cell death in vitro remained increased for at least 1 week after admission. Taken together, the data are consistent with the concept of malaria-induced reallocation of activated T cells to sites of inflammation, followed by their release back into the peripheral blood where they undergo apoptotic death to re-establish immunological homeostasis as inflammation subsides. However, the high proportion of pre-apoptotic cells from the time of admission suggests that apoptosis also contributes to the low frequency and number of T cells in the peripheral circulation during active disease.
AB - Available evidence suggests that Plasmodium falciparum malaria causes activation and reallocation of T cells, and that these in vivo primed cells re-emerge into the periphery following drug therapy. Here we have examined the cytokine production capacity and susceptibility to programmed cell death of peripheral T cells during and after the period of antimalarial treatment. A high proportion of peripheral CD3+ cells had an activated phenotype at and shortly after time of admission (day 0) and initiation of therapy. This activation peaked around day 2, and at this time-point peripheral T cells from the patients could be induced to produce cytokines at conditions of limited cytokine response in cells from healthy control donors. Activated CD8hi and TCR-gammadelta+ cells were the primary IFN-gamma producers, whereas CD4+ cells constituted an important source of TNF-alpha. The proportion of apoptotic T cells was elevated at admission and peaked 2 days later, while susceptibility to activation-induced cell death in vitro remained increased for at least 1 week after admission. Taken together, the data are consistent with the concept of malaria-induced reallocation of activated T cells to sites of inflammation, followed by their release back into the peripheral blood where they undergo apoptotic death to re-establish immunological homeostasis as inflammation subsides. However, the high proportion of pre-apoptotic cells from the time of admission suggests that apoptosis also contributes to the low frequency and number of T cells in the peripheral circulation during active disease.
M3 - Journal article
C2 - 11882046
VL - 127
SP - 151
EP - 157
JO - Clinical and Experimental Immunology, Supplement
JF - Clinical and Experimental Immunology, Supplement
SN - 0964-2536
IS - 1
ER -
ID: 6747115