Activation of human T lymphocytes by Leishmania lipophosphoglycan
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Activation of human T lymphocytes by Leishmania lipophosphoglycan. / Kemp, M; Theander, T G; Handman, E; Hey, A S; Kurtzhals, J A; Hviid, L; Sørensen, A L; Were, J O; Koech, D K; Kharazmi, A.
In: Scandinavian Journal of Immunology, Vol. 33, No. 2, 1991, p. 219-24.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Activation of human T lymphocytes by Leishmania lipophosphoglycan
AU - Kemp, M
AU - Theander, T G
AU - Handman, E
AU - Hey, A S
AU - Kurtzhals, J A
AU - Hviid, L
AU - Sørensen, A L
AU - Were, J O
AU - Koech, D K
AU - Kharazmi, A
N1 - Keywords: Animals; Antigens, Protozoan; Antinematodal Agents; Glycosphingolipids; Humans; Immunophenotyping; Interferon Type II; Leishmania tropica; Leishmaniasis, Visceral; Lymphocyte Activation; Metalloendopeptidases; Protozoan Proteins; T-Lymphocytes
PY - 1991
Y1 - 1991
N2 - This study describes Leishmania antigen-induced activation of lymphocytes isolated from Kenyan donors, previously treated for visceral leishmaniasis, and from Danish and Kenyan controls. Peripheral blood mononuclear cells (PBMC) from cured Kala-Azar patients proliferated and produced Interferon-gamma in vitro in response to lipophosphoglycan (LPG) isolated from Leishmania major. The proliferative response was mainly due to activation of CD2-positive T cells. PBMC from controls did not respond to LPG, but to sonicates prepared from both L. major and L. donovani promastigotes. The surface glycoprotein GP 63 failed to activate PBMC from any of the donors tested. These results show that the individuals cured from visceral leishmaniasis had expanded T-cell clones recognizing LPG, conceivably as a result of Leishmania infection. The LPG preparation was without detectable protein contamination. Thus, the results suggest that human T lymphocytes can respond to glycolipid antigens.
AB - This study describes Leishmania antigen-induced activation of lymphocytes isolated from Kenyan donors, previously treated for visceral leishmaniasis, and from Danish and Kenyan controls. Peripheral blood mononuclear cells (PBMC) from cured Kala-Azar patients proliferated and produced Interferon-gamma in vitro in response to lipophosphoglycan (LPG) isolated from Leishmania major. The proliferative response was mainly due to activation of CD2-positive T cells. PBMC from controls did not respond to LPG, but to sonicates prepared from both L. major and L. donovani promastigotes. The surface glycoprotein GP 63 failed to activate PBMC from any of the donors tested. These results show that the individuals cured from visceral leishmaniasis had expanded T-cell clones recognizing LPG, conceivably as a result of Leishmania infection. The LPG preparation was without detectable protein contamination. Thus, the results suggest that human T lymphocytes can respond to glycolipid antigens.
M3 - Journal article
C2 - 1902000
VL - 33
SP - 219
EP - 224
JO - Scandinavian Journal of Immunology, Supplement
JF - Scandinavian Journal of Immunology, Supplement
SN - 0301-6323
IS - 2
ER -
ID: 6748534